Signal Transduction Reagents and Kits
Promega PAK4 Kinase Enzyme System, 1mg
Recombinant full-length human PAK4 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. PAK4 is a member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility and transformation.
Promega JNK3 Kinase Enzyme System, 10microg
Recombinant full-length human JNK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK3 is a member of the c-Jun N-terminal kinases (JNKs), which are part of the mitogen-activated protein (MAP) kinase family.
Promega JNK1 Kinase Enzyme System, 10microg
Recombinant full-length human JNK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at the thr and tyr residues during exposure to stress.
Promega NIK Kinase Enzyme System, 10microg
Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.
Promega DNA-PK Kinase Enzyme System, 2,500 units
Native human DNA-Dependent Protein Kinase purified from HeLa cells. Consists of a ∼460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and a 70kDa peptide (1). Gene sequence can be found at accession number NM_006904.
Promega PRKG1 (PKG) Kinase Enzyme System, 10microg
Recombinant full-length human PRKG1 (PKG) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain.
Promega LRRK2 Kinase Enzyme System, 10microg
LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain and a WD40 domain.
Promega ADP-Glo™ Kinase Assay + c-KIT Kinase Enzyme System
Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
Promega ADP-Glo™ Kinase Assay + IKKβ Kinase Enzyme System
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + EGFR (T790M) Kinase Enzyme System
Recombinant human EGFR (T790M) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega ADP-Glo™ Kinase Assay + EGFR (L858R) Kinase Enzyme System
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega EIF2AK2 Kinase Enzyme System, 10microg
Recombinant human EIF2AK2 (252-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EIF2AK2 (also known as double-stranded RNA-activated protein kinase) is shown to be involved in HIV/gp120-associated neurodegeneration.
Promega AMPK (A2/B1/G1) Kinase Enzyme System, 1mg
Recombinant full-length human AMPK (A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for diabetes treatment.
Promega NIK Kinase Enzyme System, 1mg
Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.
Promega TAK1-TAB1 Kinase Enzyme System, 1mg
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.