Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + CDK5/p25 Kinase Enzyme System
Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega Caspase-Glo 1 Inflammasome Assay, 10ml
The Caspase-Glo 1 Inflammasome Assay is a homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome.
Promega Glutamine/Glutamate-Glo Assay, 5ml
The Glutamine/Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. The assay is based on the conversion of glutamine to glutamate by Glutaminase enzyme.
Promega CAMK2 alpha Kinase Enzyme System, 1mg
Recombinant full-length human CAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 alpha is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family.
Promega PYK2 Kinase Enzyme System, 10microg
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega ADP-Glo™ Kinase Assay + RIPK2 Kinase Enzyme System
RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain.
Promega ADP-Glo™ Kinase Assay + PKCε Kinase Enzyme System
Recombinant full-length human PKC epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC epsilon is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega CK1 gamma 1 Kinase Enzyme System, 10microg
Recombinant human CK1 gamma 1 (21-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega SRC Kinase Enzyme System, 1mg
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates.
Promega PASK Kinase Enzyme System, 1mg
Recombinant human PASK (981-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK or PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress.
Promega ADP-Glo™ Kinase Assay + CDK9/CyclinK Kinase Enzyme System
Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.
Promega p70S6Kb Kinase Enzyme System, 1mg
Recombinant full-length human p70S6Kb was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli.
Promega MELK Kinase Enzyme System, 10microg
Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MELK or maternal embryonic leucine zipper kinase is a member of the Snf1/AMPK kinase family.
Promega MELK Kinase Enzyme System, 1mg
Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MELK or maternal embryonic leucine zipper kinase is a member of the Snf1/AMPK kinase family.
Promega RIPK2 Kinase Enzyme System, 1mg
RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain.